Anti-SQSTM1 / p62 antibody
| Name | Anti-SQSTM1 / p62 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab64134 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P IHC-F ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | A synthetic peptide as a part of SQSTM1/ p62 conjugated to immunogenic carrier protein |
| Description | Rabbit Polyclonal |
| Gene | SQSTM1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of ab64134 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64134, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab64134 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64134, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Important role of autophagy in endothelial cell response to ionizing radiation. - Important role of autophagy in endothelial cell response to ionizing radiation.
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Co-inhibition of BCL-W and BCL2 restores antiestrogen sensitivity through BECN1 - Co-inhibition of BCL-W and BCL2 restores antiestrogen sensitivity through BECN1
Crawford AC, Riggins RB, Shajahan AN, Zwart A, Clarke R. PLoS One. 2010 Jan 6;5(1):e8604.
BCL2 and CASP8 regulation by NF-kappaB differentially affect mitochondrial - BCL2 and CASP8 regulation by NF-kappaB differentially affect mitochondrial
Nehra R, Riggins RB, Shajahan AN, Zwart A, Crawford AC, Clarke R. FASEB J. 2010 Jun;24(6):2040-55.
IFNgamma restores breast cancer sensitivity to fulvestrant by regulating STAT1, - IFNgamma restores breast cancer sensitivity to fulvestrant by regulating STAT1,
Ning Y, Riggins RB, Mulla JE, Chung H, Zwart A, Clarke R. Mol Cancer Ther. 2010 May;9(5):1274-85.