Anti-SQSTM1 / p62 antibody
| Name | Anti-SQSTM1 / p62 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab31545 |
| Prices | $382.00 |
| Sizes | 100 µl |
| Host | Sheep |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IHC-F WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide from part of human Ubiquitin Interactive Motif (UIM) derived from p62 protein, also known as SQSTM1 |
| Description | Sheep Polyclonal |
| Gene | SQSTM1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-SQSTM1 / p62 antibody (ab31545) at 1/500 dilutionLane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateLane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryRabbit polyclonal Secondary Antibody to Sheep IgG - H&L (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab31545 staining SQSTM1 / p62 in murine spinal cord sections by Immunohistochemistry (Frozen sections).Tissue was fixed in 4% paraformaldehyde, blocked with 10% goat serum for 2 hours at 25°C and then incubated with ab31545 at a 1/500 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated donkey anti-sheep (H+L) polyclonal used at a 1/1000 dilution.See Abreview
Product References
CIP2A oncoprotein controls cell growth and autophagy through mTORC1 activation. - CIP2A oncoprotein controls cell growth and autophagy through mTORC1 activation.
Puustinen P, Rytter A, Mortensen M, Kohonen P, Moreira JM, Jaattela M. J Cell Biol. 2014 Mar 3;204(5):713-27.
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Castillo K, Rojas-Rivera D, Lisbona F, Caballero B, Nassif M, Court FA, Schuck S, Ibar C, Walter P, Sierralta J, Glavic A, Hetz C. EMBO J. 2011 Sep 16;30(21):4465-78.