Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

• Supplier: Abcam
• Catalog: ab109012
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4844
• Applications: WB ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide designed within the sequence of human SQSTM1
• Description: Rabbit Monoclonal
• Gene: SQSTM1
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab109012 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109012, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (ab109012)Lane 1 : MCF-7Lane 2 : HeLaLane 3 : SKBR-3Lane 4 : 293TLysates/proteins at 10 µg per lane.Observed band size : 62 kDa (why is the actual band size different from the predicted?)

ab109012 staining of SQSTM1/p62 by immunofluorescence

Product References

Mitophagy enhances oncolytic measles virus replication by mitigating - Mitophagy enhances oncolytic measles virus replication by mitigating

Xia M, Gonzalez P, Li C, Meng G, Jiang A, Wang H, Gao Q, Debatin KM, Beltinger C, Wei J. J Virol. 2014 May;88(9):5152-64.