Anti-SREBP1 antibody

• Supplier: Abcam
• Catalog: ab28481
• Prices: $392.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IHC-F IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide: MLQLINNQDSDFPGLF , corresponding to amino acids 32-47 of Mouse SREBP 1 Run BLAST with Run BLAST with
• Blocking Peptide: Mouse SREBP1 peptide
• Description: Rabbit Polyclonal
• Gene: SREBF1
• Conjugate: Unconjugated

Product images

ab28481 staining SREBP1 in mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 15 minutes at 25°C. Samples were incubated with primary antibody (1/2000 in goat serum) for 12 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview

ICC/IF image of ab28481 stained HepG2 cells. The cells were 10% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28481, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, goat anti rabbit DyLight® 488 IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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Reduced VLDL clearance in Apoe(-/-)Npc1(-/-) mice is associated with increased - Reduced VLDL clearance in Apoe(-/-)Npc1(-/-) mice is associated with increased

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Honokiol reverses alcoholic fatty liver by inhibiting the maturation of sterol - Honokiol reverses alcoholic fatty liver by inhibiting the maturation of sterol

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