SREBF1

Gene Symbol SREBF1
Entrez Gene 6720
Alt Symbol SREBP-1c, SREBP1, bHLHd1
Species Human
Gene Type protein-coding
Description sterol regulatory element binding transcription factor 1
Other Description SREBP-1|class D basic helix-loop-helix protein 1|sterol regulatory element-binding protein 1
Swissprots B0I4X4 Q6PFW7 Q16062 Q8TAK9 P36956 Q59F52 Q6PJ36 B0I4X3 D3DXC4 Q6P4R7
Accessions EAW55688 EAW55689 EAW55690 EAW55691 P36956 AB209609 BAD92846 AB373958 BAG06742 AB373959 BAG06743 AK091131 BAG52289 AK095325 BAG53027 AK128320 BAG54659 AK293795 BAG57207 AK294800 BAG57920 AK297113 BAG59620 BC023621 AAH23621 BC026962 AAH26962 BC057388 AAH57388 BC063281 AAH63281 BE208013 BI906407 BQ025047 S66167 AAB28522 S66168 AAB28523 U00968 AAC50051 XM_005256772 XP_005256829 XM_006721570 XP_006721633 XM_011523998 XP_011522300 XM_011523999 XP_011522301 XR_429821 NM_001005291 NP_001005291 NM_004176 NP_004167
Function Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). {ECO:0000250|UniProtKB:Q9WTN3}.
Subcellular Location Isoform SREBP-1aDelta: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981, ECO:0000269|PubMed:18267114}.
Tissue Specificity Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
Top Pathways Insulin signaling pathway, Non-alcoholic fatty liver disease (NAFLD), AMPK signaling pathway

Phospho-SREBP-1c (Ser372) Antibody - 9874 from Cell Signaling Technology

Prices $279.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from serum-starved 293T cells treated with resveratrol (50 μM, 1 hr), transfected with a GST-tagged cDNA expression construct encoding either full-length human wild-type SREBP-1c (GST-hSREBP-1c) or full-length mutant SREBP-1c (GST-hSREBP-1c (S372A)), using Phospho-SREBP-1c (Ser372) Antibody (upper) or GST (91G1) Rabbit mAb #2625 (lower). Both expression vectors were generated in Dr. Mengwei Zang's laboratory at Boston University School of Medicine.

SREBP-1 (A-4) - sc-365513 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SREBP-1 (A-4): sc-365513. Western blot analysis of SREBP-1 expression in non-transfected: sc-117752 (A) and human SREBP-1 transfected: sc-116164 (B) 293T whole cell lysates and NIH/3T3 nuclear extract (C).

SREBP-1 (C-20) - sc-366 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SREBP-1 (C-20): sc-366. Western blot analysis of SREBP-1 expression in NIH/3T3 nuclear extract (A) and KNRK (B), LNCaP (C) and Hep G2 (D) whole cell lysates.

SREBP-1 (E-4) - sc-17755 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SREBP-1 (E-4): sc-17755. Western blot analysis of SREBP-1 precursor expression in HeLa nuclear extract.

SREBP-1 (F-10) - sc-365514 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SREBP-1 (F-10): sc-365514. Western blot analysis of SREBP-1 expression in non-transfected: sc-117752 (A) and human SREBP-1 transfected: sc-116164 (B) 293T whole cell lysates.

SREBP-1 (H-160) - sc-8984 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SREBP-1 (H-160): sc-8984. Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse liver tissue (A) and immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization (B).

SREBP-1 (K-10) - sc-367 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SREBP-1 (K-10): sc-367. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human ovary showing membrane staining.

SREBP-1 (2A4) - sc-13551 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SREBP-1 siRNA (h): sc-36557. Western blot analysis of SREBP-1 expression in non-transfected control (A) and SREBP-1 siRNA transfected (B) HeLa cells. Blot probed with SREBP-1 (2A4): sc-13551. α-actinin (H-2): sc-17829 used as specificity and loading control.

SREBP-1 (SPM449) - sc-56483 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SREBP-1 (SPM449): sc-56483. Western blot analysis of SREBP-1 expression in LNCaP whole cell lysate.

SREBP-1 (3F335) - sc-73058 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Anti-SREBP1 (phospho S439) antibody - ab138663 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-SREBP1 (phospho S439) antibody (ab138663) at 1/500 dilution + Jurkat cells extracts (treated with TNF 20ng/ml for 30 mins) at 30 µg

Anti-SREBP1 (phospho S439) antibody - ab192226 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SREBP1 (phospho S439) antibody (ab192226) at 1/500 dilutionLane 1 : TNF treated Jurkat whole cell lysate with antigen-specific peptideLane 2 : TNF treated Jurkat whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-SREBP1 antibody - ab28481 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab28481 staining SREBP1 in mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 15 minutes at 25°C. Samples were incubated with primary antibody (1/2000 in goat serum) for 12 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview

Anti-SREBP1 antibody - ab191857 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SREBP1 antibody (ab191857) at 1 µg/mlLane 1 : Daudi cell lysateLane 2 : Daudi cell lysateLysates/proteins at 15 µg per lane.

Anti-SREBP1 antibody - ab193318 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SREBP1 antibody (ab193318) at 1/500 dilutionLane 1 : Jurkat cell extract treated with TNF alphaLane 2 : Jurkat cell extract treated with TNF alpha with antigen-specific peptide

Anti-SREBP1 antibody - ab93333 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-SREBP1 antibody (ab93333) at 1/100 dilution + MDAMB435 cell line lysates at 35 µg

Anti-SREBP1 antibody - ab93638 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab93638 at 1/100 dilution, showing membrane staining of SREBP1 in formalin-fixed, paraffin-embedded Human liver tissue by Immunohistochemistry.

Anti-SREBP1 antibody - ab57999 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-SREBP1 antibody - ab58000 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-SREBP1 antibody [2A4] - ab3259 from Abcam

Prices $400.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone 2A4
ICC/IF image of ab3259 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3259, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SREBP1 antibody - N-terminal - ab135133 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC image of SREBP1 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab135133, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SREBP1c (phospho S372) antibody - ab140483 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SREBP1c (phospho S372) antibody (ab140483) at 1 µg/mlLane 1 : Human Fetal Liver Tissue LysateLane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 3 : Human Fetal Liver Tissue Lysate with Immunising peptide at 1 µg/mlLane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 5 : Human Fetal Liver Tissue Lysate with Control peptide at 1 µg/mlLane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Control peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-SREBF1 / SREBP-1 Antibody (Internal) - LS-C336176 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of SREBF1 in human spleen tissue with SREBF1 antibody at 5 ug/mL.

Anti-SREBF1 / SREBP-1 Antibody - LS-C147073 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SREBF1 / SREBP-1 Antibody (Internal) - LS-C137085 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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