Anti-SRGAP1 antibody
| Name | Anti-SRGAP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57504 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment: PLDPETIAQD IEETMNTALN ELRELERQST AKHAPDVVLD TLEQVKNSPT PATSTESLSP LHNVALRSSE PQIRRSTSSS SDTMSTFKPM VAPRMGVQL, corresponding to amino acids 952-1051 of Human SRGAP1 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | SRGAP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab57504 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab57504 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
SRGAP1 antibody (ab57504) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
ab57504 staining SRGAP1 in Human Fetal Brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized with 0.1% Triton-X-100 i PBS; antigen retrieval was by heat mediation with a citrate buffer (10mM). Samples were incubated with primary antibody (1/900 in 3% serum/0.1% Triton-X-100 in PBS) for 16 hours at 4°C. A Biotin-conjugated Horse anti-mouse polyclonal (1/200) was used as the secondary antibody.See Abreview
ab57504 staining SRGAP1 in human fetal brainstem tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton X-100/PBS. Samples were then incubated with 3% serum and the primary antibody at a 1/1000 dilution, for 16 hours at 4°C. A biotin-conjugated horse anti-mouse IgG polyclonal was used as the secondary antibody at a 1/200 dilution.See Abreview
![Overlay histogram showing SH-SY5Y cells stained with ab57504 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57504, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/4965_SRGAP1-Primary-antibodies-ab57504-9.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab57504 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57504, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Slit-Robo GTPase-activating proteins are differentially expressed in murine - Slit-Robo GTPase-activating proteins are differentially expressed in murine
Chen ZB, Zhang HY, Zhao JH, Zhao W, Zhao D, Zheng LF, Zhang XF, Liao XP, Yi XN. Anat Rec (Hoboken). 2012 Apr;295(4):652-60.
The corticofugal neuron-associated genes ROBO1, SRGAP1, and CTIP2 exhibit an - The corticofugal neuron-associated genes ROBO1, SRGAP1, and CTIP2 exhibit an
Ip BK, Bayatti N, Howard NJ, Lindsay S, Clowry GJ. Cereb Cortex. 2011 Jun;21(6):1395-407.