Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121977 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-srGAP2 antibody (ab121977) at 1/100 dilutionLane 1 : COS7 Whole cell lysateLane 2 : HEK293H Whole Cell LysateLane 3 : HEK293H Over expressing human srGAP1Lane 4 : HEK293H Over expressing human srGAP2Lane 5 : HEK293H Over expressing human srGAP3Lane 6 : HeLa cells over expressing mouse srGAP2Lane 7 : Mouse E14 hindbrain lysateLysates/proteins at 30 µg per lane.SecondaryGoat polyclonal to Rabbit IgG Conjugated to HRP at 1/20000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab121977, at 1/10 dilution, staining srGAP2 in paraffin-embedded Human adrenal gland tissue by Immunohistochemistry.