All lanes : Anti-SSPN antibody [EPR15515] (ab186730) at 1/10000 dilutionLane 1 : Human fetal heart tissue lysateLane 2 : Human skeletal muscle tissue lysateLane 3 : A673 cell lysateLane 4 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-SSPN antibody [EPR15515] (ab186730) at 1/1000 dilutionLane 1 : Mouse heart tissue lysateLane 2 : Rat brain tissue lysateLane 3 : Rat heart tissue lysateLane 4 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution