Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or LPS-treated (1 μg/ml, 24 hr; +), using Cox2 (D5H5) XP ® Rabbit mAb or β-Actin (D6A8) Rabbit mAb #8457.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Cox2 (D5H5) XP ® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Cox2 (D5H5) XP ® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Confocal immunofluorescent analysis of Raw 264.7 cells, untreated (left; -) or LPS-treated (right; 1ug/ml, 24 hrs; +), using Cox2 (D5H5) XP ® Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or LPS-treated (1μg/ml, 24 hrs; green), using Cox2 (D5H5) XP ® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.