PTGS2

Gene Symbol PTGS2
Entrez Gene 5743
Alt Symbol COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2
Species Human
Gene Type protein-coding
Description prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Other Description PGH synthase 2|PHS II|cyclooxygenase 2b|prostaglandin G/H synthase 2|prostaglandin H2 synthase 2
Swissprots Q16876 P35354 A8K802
Accessions AAA57317 AAD14842 AAF85977 AAN87129 AAO38056 AAQ75702 BAA05698 CBH19826 CBX54355 EAW91216 P35354 AJ634912 CAG25548 AK292167 BAF84856 AY151286 AAN52932 AY462100 AAR23927 BC013734 AAH13734 BM978796 DA277667 EU176282 ABW03733 EU176597 ABW03398 HM473188 ADJ56342 L15326 AAA35803 M90100 AAA58433 NM_000963 NP_000954
Function Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. {ECO:0000269|PubMed:16373578}.
Subcellular Location Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.
Top Pathways Leishmaniasis, Chemical carcinogenesis, Retrograde endocannabinoid signaling, TNF signaling pathway, Ovarian steroidogenesis

Cox2 (D5H5) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 13596 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D5H5
Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or LPS-treated (1 μg/ml, 24 hr; green), using Cox2 (D5H5) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate).

Cox2 (D5H5) XP ® Rabbit mAb (PE Conjugate) - 13314 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D5H5
Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or LPS-treated (1 µg/ml, 24 hr; green), using Cox2 (D5H5) XP ® Rabbit mAb (PE Conjugate).

Cox2 (D5H5) XP ® Rabbit mAb - 12282 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5H5
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or LPS-treated (1 μg/ml, 24 hr; +), using Cox2 (D5H5) XP ® Rabbit mAb or β-Actin (D6A8) Rabbit mAb #8457.

Cox2 Antibody - 4842 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot of extracts from Raw264.7 cells, untreated or LPS-treated (1 µg/ml for 6 h), using Cox2 Antibody.

Cox-2 (H-3) - sc-376861 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cox-2 (H-3): sc-376861. Western blot analysis of Cox-2 expression in LPS/PMA treated RAW 264.7 whole cell lysate.

Cox-2 (H-62) - sc-7951 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cox-2 (H-62): sc-7951. Western blot analysis of Cox-2 expression in PMA + LPS-induced RAW 264.7 whole cell lysate.

Cox-2 (M-19) - sc-1747 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cox-2 (M-19): sc-1747. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor showing membrane and cytoplasmic staining (A). Immunofluorescence staining of methanol-fixed RAW 264.7 cells induced with LPS and PMA showing cytoplasmic vesicle localization (B) and untreated control RAW 264.7 cells (C).

Cox-2 (N-20) - sc-1746 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cox-2 (N-20): sc-1746. Western blot analysis of Cox-2 expression in uninduced (A) and LPS + PMA-treated (B) RAW 264.7 whole cell lysates.

Cox-2 (D-12) - sc-166475 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cox-2 (D-12): sc-166475. Western blot analysis of Cox-2 expression in non-transfected: sc-110760 (A) and human Cox-2 transfected: sc-113099 (B) 293 whole cell lysates.

Cox-2 (C-20) - sc-1745 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of Cox-2 expression in uninduced (A,C) and LPS + PMA-treated (B,D) RAW 264.7 whole cell lysates. Antibodies tested include Cox-2 (C-20): sc-1745 (A,B) and Cox-2 (M-19): sc-1747 (C,D).

Cox-2 (29) - sc-19999 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cox-2 (29): sc-19999. Western blot analysis of Cox-2 expression in non-transfected: sc-110760 (A) and human Cox-2 transfected: sc-113099 (B) 293 whole cell lysates.

Cox-2 (4i271) - sc-70879 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Cox-2 (4i271): sc-70879. Western blot analysis of Cox-2 expression in non-transfected: sc-110760 (A) and human Cox-2 transfected: sc-113099 (B) 293 whole cell lysates.

Anti-COX2 / Cyclooxygenase 2 antibody - ab15191 from Abcam

Prices $400.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ab15191 staining COX2 in HepG2 cells treated with sinomenine hydrochloride (ab141190), by ICC/IF. Decrease of COX2 expression correlates with increased concentration of sinomenine hydrochloride, as described in literature.The cells were incubated at 37°C for 48 hours in media containing different concentrations of ab141190 (sinomenine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab15191 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Anti-COX2 / Cyclooxygenase 2 antibody - ab52237 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of COX2 / Cyclooxygenase 2 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52237, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-COX2 / Cyclooxygenase 2 antibody - ab6665 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-COX2 / Cyclooxygenase 2 antibody - ab81354 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab81354 at 1µg/ml staining cox2 / Cyclooxygenase 2 in Human mammary cancer tissue sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded sections). A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.

Anti-COX2 / Cyclooxygenase 2 antibody - ab88522 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-COX2 / Cyclooxygenase 2 antibody (ab88522) at 1 µg/ml + Jurkat cell lysate at 50 µg

Anti-COX2 / Cyclooxygenase 2 antibody [4H12] - ab10940 from Abcam

Host Mouse
Clonality Monoclonal
Clone 4H12
Formalin fixed paraffin embedded human colon carcinoma tissue stained with ab10940 (anti-Cox-2 mouse monoclonal antibody).

Anti-COX2 / Cyclooxygenase 2 antibody [5E10/D10] - ab701 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5E10/D10
Lane 1: Cox-2 (protein standard prepared from ovine placenta, supplied by Alpha Diagnostic International).Lane 2: MM standardAntibody 5E10/D10: 1 µg/ml

Anti-COX2 / Cyclooxygenase 2 antibody [AS66] - ab90345 from Abcam

Prices $379.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone AS66
Standard Curve for COX2 / Cyclooxygenase 2 (Analyte: ab58868) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS66] to COX2 / Cyclooxygenase 2 (ab90345) at 1ug/ml and Detector Antibody Rabbit polyclonal to COX2 / Cyclooxygenase 2 (ab15191) at 0.5ug/ml

Anti-COX2 / Cyclooxygenase 2 antibody [AS66] (FITC) - ab16117 from Abcam

Prices $370.00
Sizes 100 tests
Host Mouse
Clonality Monoclonal
Clone AS66

Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] - ab62331 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1978Y
ab62331 staining COX2 / Cyclooxygenase 2 in Mouse 14.5 dpc tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with TBS-T and blocked with 1% BSA + 1% FBS in TBS for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris buffer, pH9. Samples were incubated with primary antibody (1/100 in blocking buffer) for 16 hours. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview

Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (Alexa Fluor® 594) - ab202266 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1978Y
ab202266 staining COX2/Cyclooxygenase 2 in SKNSH cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202266 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-COX2 / Cyclooxygenase 2 antibody [EPR468(2)] - ab133466 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR468(2)
All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EPR468(2)] (ab133466) at 1/1000 dilutionLane 1 : A549 treated with TNF-alpha and TPALane 2 : A549 cell lysate

Anti-COX2 / Cyclooxygenase 2 antibody [EPR8588] - ab151571 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8588
Anti-COX2 / Cyclooxygenase 2 antibody [EPR8588] (ab151571) at 1/1000 dilution + A549 cell lysate at 10 µgSecondaryGoat anti-rabbit HRP at 1/2000 dilution
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