Anti-STAT3 (phospho Y705) antibody [EP2147Y]

Name	Anti-STAT3 (phospho Y705) antibody [EP2147Y]
Supplier	Abcam
Catalog	ab76315
Prices	$99.00, $403.00
Sizes	10 µl, 100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EP2147Y
Applications	WB IP IHC-P ICC/IF ICC/IF
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human STAT3 (phospho Y705)
Blocking Peptide	STAT3 (phospho Y705) peptide
Description	Rabbit Monoclonal
Gene	STAT3
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315) at 1/200000 dilution (purified)Lane 1 : HeLa cell lysate - untreatedLane 2 : HeLa cell lysate - treated with IFN-aLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315) at 1/20000 dilution (unpurified)Lane 1 : HeLa cell lysateLane 2 : HeLa cell lysate, treated with alpha interferonLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit-HRP at 1/1000 dilutiondeveloped using the ECL technique

developed using the ECL techniquePerformed under reducing conditions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling STAT3 (phospho Y705) with purified ab76315 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling STAT3 (phospho Y705) with unpurified ab76315 at 1/100.

Immunocytochemistry/Immunofluorescence analysis of HeLa cells, treated with IFN-a or non-treated, labelling STAT3 (phospho Y705) with purified ab76315 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Controls: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling STAT3 (phospho Y705) with unpurified ab76315 at 1/100 dilution.

Unpurified ab76315 staining STAT3 (phospho Y705) in MDA-MB-231 cells treated with stattic (ab120952), by ICC/IF. Decrease in STAT3 (phospho Y705) expression correlates with increased concentration of stattic as described in literature.The cells were incubated at 37°C for 2h in media containing different concentrations of ab120952 (stattic) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with unpurified ab76315 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

ab76315 (purified) at 1/30 immunoprecipitating STAT3 (phospho Y705) in A431 cell lysate treated with EGF. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

Product References

EGFR-STAT3 signaling promotes formation of malignant peripheral nerve sheath - EGFR-STAT3 signaling promotes formation of malignant peripheral nerve sheath

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In silico simulations of STAT1 and STAT3 inhibitors predict SH2 domain - In silico simulations of STAT1 and STAT3 inhibitors predict SH2 domain

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A TrkB-STAT3-miR-204-5p regulatory circuitry controls proliferation and invasion - A TrkB-STAT3-miR-204-5p regulatory circuitry controls proliferation and invasion

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Generation of prostate tumor-initiating cells is associated with elevation of - Generation of prostate tumor-initiating cells is associated with elevation of

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