Anti-Stathmin 1 (phospho S37) antibody

• Supplier: Abcam
• Catalog: ab47399
• Prices: $370.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB ELISA IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthesized phosphopeptide derived from human Stathmin 1 around the phosphorylation site of serine 37 (P-L-SP-P-P)
• Description: Rabbit Polyclonal
• Gene: STMN1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Stathmin 1 (phospho S37) antibody (ab47399) at 1/500 dilutionLane 1 : Extracts from Jurkat cells treated with PMA (1ng/ml, 15min) Lane 2 : Extracts from Jurkat cells untreated

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47399 at a 1/50 dilution. Left image: Untreated Right image: Treated with phosphopeptide

ICC/IF image of ab47399 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47399, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

cAMP-dependent protein kinase and c-Jun N-terminal kinase mediate stathmin - cAMP-dependent protein kinase and c-Jun N-terminal kinase mediate stathmin

Yip YY, Yeap YY, Bogoyevitch MA, Ng DC. J Biol Chem. 2014 Jan 24;289(4):2157-69.

WAVE2 targeting to phosphatidylinositol 3,4,5-triphosphate mediated by insulin - WAVE2 targeting to phosphatidylinositol 3,4,5-triphosphate mediated by insulin

Takahashi K, Suzuki K. Cell Signal. 2010 Nov;22(11):1708-16.