Anti-Stathmin 1 antibody
| Name | Anti-Stathmin 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab52906 |
| Prices | $391.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP FC IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues in N terminus of human Stathmin |
| Description | Rabbit Polyclonal |
| Gene | STMN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Stathmin 1 was immunoprecipitated using 0.5mg PC12 whole cell extract, 5µg of Rabbit polyclonal to Stathmin 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, PC12 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52906.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 17kDa; Stathmin 1](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/5983_ab52906-195101-IPV020ab5290620mMod.jpg)
Stathmin 1 was immunoprecipitated using 0.5mg PC12 whole cell extract, 5µg of Rabbit polyclonal to Stathmin 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, PC12 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52906.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 17kDa; Stathmin 1
Anti-Stathmin 1 antibody (ab52906) at 1/50000 dilution + PC12 cell lysate at 10 µg
Immunofluorescent staining of Stathmin 1 in HeLa cells using ab52906 at 1/100 dilution.
Immunohistochemical analysis of Stathmin 1 in paraffin embedded human tonsil tissue using ab52906 at 1/250 dilution.Immunohistochemical analysis of Stathmin 1 in paraffin embedded human tonsil tissue using ab52906 at 1/250 dilution.
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