All lanes : Anti-STEAP2 antibody (ab174978) at 1/100 dilutionLane 1 : Mouse spleen tissue lysateLane 2 : Mouse liver tissue lysateLane 3 : 293 cell lysateLysates/proteins at 15 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling STEAP2 with ab174978 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometry analysis of 293 cells labeling STEAP2 with ab174978 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.