Anti-STIL antibody
| Name | Anti-STIL antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab89314 |
| Prices | $370.00 |
| Sizes | 20 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP |
| Species Reactivities | Mouse, Human, Rat, Rabbit, Horse, Guinea Pig, Bovine, Pig, Chimpanzee, Ape, Opossum, Orangutan, Elephant |
| Antigen | Synthetic peptide corresponding to a region between residue 1237 and 1287 of Human STIL (NP_003026 |
| Description | Rabbit Polyclonal |
| Gene | STIL |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-STIL antibody (ab89314) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique
Detection of STIL in Immunoprecipitates of Hela whole cell lysates (1 mg for IP, 20% of IP loaded) using ab89314 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with exposure time of 30 seconds.
ICC/IF image of ab89314 stained T24/83 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89314, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Cell-cycle-regulated expression of STIL controls centriole number in human cells. - Cell-cycle-regulated expression of STIL controls centriole number in human cells.
Arquint C, Sonnen KF, Stierhof YD, Nigg EA. J Cell Sci. 2012 Mar 1;125(Pt 5):1342-52.
VIP blockade leads to microcephaly in mice via disruption of Mcph1-Chk1 - VIP blockade leads to microcephaly in mice via disruption of Mcph1-Chk1
Passemard S, El Ghouzzi V, Nasser H, Verney C, Vodjdani G, Lacaud A, Lebon S, Laburthe M, Robberecht P, Nardelli J, Mani S, Verloes A, Gressens P, Lelievre V. J Clin Invest. 2011 Aug;121(8):3071-87.