Anti-STIM2 antibody
| Name | Anti-STIM2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab59342 |
| Prices | $388.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF ELISA |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | An 18 amino acid peptide from near the carboxy terminus of human STIM2 |
| Blocking Peptide | STIM2 peptide |
| Description | Rabbit Polyclonal |
| Gene | STIM2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-STIM2 antibody (ab59342) at 0.5 µg/mlLane 2 : Anti-STIM2 antibody (ab59342) at 1 µg/mlLane 3 : Anti-STIM2 antibody (ab59342) at 2 µg/mlLane 1 : A-20 cell lysateLane 2 : A-20 cell lysateLane 3 : A-20 cell lysateLysates/proteins at 15 µg per lane.
ICC/IF image of ab59342 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59342, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
STIM1 but not STIM2 is an essential regulator of Ca2+ influx-mediated NADPH - STIM1 but not STIM2 is an essential regulator of Ca2+ influx-mediated NADPH
Brechard S, Plancon S, Melchior C, Tschirhart EJ. Biochem Pharmacol. 2009 Sep 1;78(5):504-13.
Dominant role of Orai1 with STIM1 on the cytosolic entry and cytotoxicity of lead - Dominant role of Orai1 with STIM1 on the cytosolic entry and cytotoxicity of lead
Chiu TY, Teng HC, Huang PC, Kao FJ, Yang DM. Toxicol Sci. 2009 Aug;110(2):353-62.