ab104560 staining STMN2 in rat glioblastoma cell line C6 by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab104560 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a Cy3 conjugated goat anti-rabbit polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.See Abreview
Immunohistochemical analysis of murine E12.5 limbs, staining STMN2 (green) with ab104560. Tissue was taken from Pitx2LacZ/+ mice (left) and Pitx2LacZ/LacZ mice (right). Tissue was fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with blocking buffer for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody. Nuclei were stained with DAPI (blue).