All lanes : Anti-STMN2 antibody [EPR15286-39] (ab185956) at 1/20000 dilutionLane 1 : Human fetal brain lysatesLane 2 : SH-SY5Y cell lysatesLane 3 : Rat brain lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunoprecipitation of Human fetal brain lysates using ab185956 (lane 1). Detection of STMN2 utilised ab185956 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. The control utilised PBS instead of Human fetal brain lysates (lane 2). Based on the sequence analysis, ab185956 recognizes two isoforms with the predicted MWs of 21KDa and 22KDa, respectively.