All lanes : Anti-STUB1 antibody [EPR4447] (ab134064) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF-7 cell lysateLane 3 : 293T cell lysateLane 4 : ECV-304 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling STUB1 with ab134064 at 1/100 dilution.
All lanes : Anti-STUB1 antibody [EPR4447] (ab134064) at 1/1000 dilutionLane 1 : Wild type primary mouse neurons whole cell lysateLane 2 : STUB1 knockout primary mouse neurons whole cell lysateLane 3 : Wild type MEFs whole cell lysateLane 4 : STUB1 knockout MEFs whole cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG polyclonal at 1/20000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.