Anti-Styk1 antibody [2H2F10]
| Name | Anti-Styk1 antibody [2H2F10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab63570 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2H2F10 |
| Applications | WB IHC-P ELISA FC |
| Species Reactivities | Human |
| Antigen | His tagged recombinant fragment expressed in E |
| Description | Mouse Monoclonal |
| Gene | STYK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab63570 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab63570, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/6585_ab63570-4-ab63570FC.jpg)
Overlay histogram showing HeLa cells stained with ab63570 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab63570, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemical analysis of paraffin embedded human normal and tumour tissue, showing cytoplasmic location using ab63570 at 1/500 dilution.
Product References
Clinicopathologic features and prognostic implications of NOK/STYK1 protein - Clinicopathologic features and prognostic implications of NOK/STYK1 protein
Chen P, Li WM, Lu Q, Wang J, Yan XL, Zhang ZP, Li XF. BMC Cancer. 2014 Jun 4;14:402.
Aberrant STYK1 expression in ovarian cancer tissues and cell lines. - Aberrant STYK1 expression in ovarian cancer tissues and cell lines.
Jackson KA, Oprea G, Handy J, Kimbro KS. J Ovarian Res. 2009 Oct 21;2(1):15.