Anti-Sumo 1 antibody
| Name | Anti-Sumo 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab11672 |
| Prices | $398.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P IP |
| Species Reactivities | Human, Escherichia coli |
| Antigen | Recombinant his-tagged full length soluble Sumo 1 (Human) |
| Description | Rabbit Polyclonal |
| Gene | SUMO1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescence of 293T cells transfected with a vector that has Sumo1 fused to GFP and a Flag tag. The top panel is GFP fluorescence. The middle panel uses ab11672 at 1/300 with a rhodamine secondary antibody. The lower panel is a merge of the GFP fluorescence and ab11672 immunostaining. Staining was also seen in untransfected cells (although at a lower level).
293T cells were transfected with a vector that has Sumo1 fused to GFP and a Flag tag. Cell lysates were used in IP with ab11672 (and a GFP antibody as a control). The resulting western blot was performed with a Flag antibody. As a control, cells were transfected with a a vector with Sumo2 fused to GFP and a Flag tag. ab11672 does not IP anything from this lysate.Lane 1: Sumo1 fusion lysate - IP'd with GFP antibodyLane 2: Sumo1 fusion lysate - no IPLane 3: Sumo1 fusion lysate - IP'd with ab11672Lane 4: Sumo2 fusion lysate - IP'd with ab11672
Ab11672 staining human normal placenta. Staining is localized to the nucleus and nuclear membrane.Left panel: with primary whole serum antibody at 1/400. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ICC/IF image of ab11672 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11672, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence analysis of WM-266-4 cells. Left panel = untreated cells. Right panel = cells treated for 3 h with 25 ng/ml leptomycin B and for 45 min with 50 ng/ml NRG-1. Sumo 1 was stained with ab11672 (green).
Product References
Sumoylation regulates ER stress response by modulating calreticulin gene - Sumoylation regulates ER stress response by modulating calreticulin gene
Lim Y, Lee D, Kalichamy K, Hong SE, Michalak M, Ahnn J, Kim do H, Lee SK. Int J Biochem Cell Biol. 2014 Aug;53:399-408.
NF-kappaB (p65) negatively regulates myocardin-induced cardiomyocyte hypertrophy - NF-kappaB (p65) negatively regulates myocardin-induced cardiomyocyte hypertrophy
Liao XH, Wang N, Zhao DW, Zheng DL, Zheng L, Xing WJ, Zhou H, Cao DS, Zhang TC. Cell Signal. 2014 Dec;26(12):2738-48.
ATR acts stage specifically to regulate multiple aspects of mammalian meiotic - ATR acts stage specifically to regulate multiple aspects of mammalian meiotic
Royo H, Prosser H, Ruzankina Y, Mahadevaiah SK, Cloutier JM, Baumann M, Fukuda T, Hoog C, Toth A, de Rooij DG, Bradley A, Brown EJ, Turner JM. Genes Dev. 2013 Jul 1;27(13):1484-94.
De-SUMOylation of CCCTC binding factor (CTCF) in hypoxic stress-induced human - De-SUMOylation of CCCTC binding factor (CTCF) in hypoxic stress-induced human
Wang J, Wang Y, Lu L. J Biol Chem. 2012 Apr 6;287(15):12469-79.
Protein inhibitor of activated STAT3 (PIAS3) protein promotes SUMOylation and - Protein inhibitor of activated STAT3 (PIAS3) protein promotes SUMOylation and
Sundvall M, Korhonen A, Vaparanta K, Anckar J, Halkilahti K, Salah Z, Aqeilan RI, Palvimo JJ, Sistonen L, Elenius K. J Biol Chem. 2012 Jun 29;287(27):23216-26.
SIRT1 stabilizes PML promoting its sumoylation. - SIRT1 stabilizes PML promoting its sumoylation.
Campagna M, Herranz D, Garcia MA, Marcos-Villar L, Gonzalez-Santamaria J, Gallego P, Gutierrez S, Collado M, Serrano M, Esteban M, Rivas C. Cell Death Differ. 2011 Jan;18(1):72-9.
53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of - 53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of
Lukas C, Savic V, Bekker-Jensen S, Doil C, Neumann B, Pedersen RS, Grofte M, Chan KL, Hickson ID, Bartek J, Lukas J. Nat Cell Biol. 2011 Mar;13(3):243-53.
Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects - Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects
Fuhs SR, Insel PA. J Biol Chem. 2011 Apr 29;286(17):14830-41.
Adenovirus E1B 55-kilodalton protein is a p53-SUMO1 E3 ligase that represses p53 - Adenovirus E1B 55-kilodalton protein is a p53-SUMO1 E3 ligase that represses p53
Pennella MA, Liu Y, Woo JL, Kim CA, Berk AJ. J Virol. 2010 Dec;84(23):12210-25.
Sumoylation of Prox1 controls its ability to induce VEGFR3 expression and - Sumoylation of Prox1 controls its ability to induce VEGFR3 expression and
Pan MR, Chang TM, Chang HC, Su JL, Wang HW, Hung WC. J Cell Sci. 2009 Sep 15;122(Pt 18):3358-64.