SUMO1

Gene Symbol SUMO1
Entrez Gene 7341
Alt Symbol DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1
Species Human
Gene Type protein-coding
Description small ubiquitin-like modifier 1
Other Description GAP modifying protein 1|SMT3 homolog 3|SMT3 suppressor of mif two 3 homolog 1|sentrin|small ubiquitin-related modifier 1|ubiquitin-homology domain protein PIC1|ubiquitin-like protein SMT3C|ubiquitin-like protein UBL1
Swissprots P55856 Q6FGG0 A8MUS8 Q93068 P63165 B2R4I5 Q6NZ62
Accessions AAY24035 EAW70304 EAW70305 EAW70306 EAW70307 P63165 AA401634 AB062294 BAB93477 AI815216 AK311840 BAG34782 BC005899 BC006462 AAH06462 BC053528 AAH53528 BC066306 AAH66306 BG503431 BG569821 BQ930026 BT006632 AAP35278 BU532734 CA313671 CR542147 CAG46944 CR542156 CAG46953 DQ893210 ABM84136 DQ896156 ABM87155 U38784 AAC50733 U61397 AAB40388 U67122 AAC50996 U72722 AAB40390 U83117 AAB39999 X99586 CAA67898 NM_001005781 NP_001005781 NM_001005782 NP_001005782 NM_003352 NP_003343
Function Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. {ECO:0000269|PubMed:18408734, ECO:0000269|PubMed:18538659, ECO:0000269|PubMed:21965678, ECO:0000269|PubMed:9019411, ECO:0000269|PubMed:9162015}.
Subcellular Location Nucleus membrane. Nucleus speckle. Cytoplasm. Nucleus, PML body. Note=Recruited by BCL11A into the nuclear body. {ECO:0000250}.
Top Pathways RNA transport

SUMO-1 (2A12) Mouse mAb - 5718 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 2A12
Western blot analysis of recombinant GST-SUMO-1 protein, His-SUMO-2 and His-SUMO-3 using SUMO-1 (2A12) Mouse mAb (upper) and SUMO-2/3 (18H8) Rabbit mAb #4971 (lower).

SUMO-1 Antibody - 4930 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of recombinant GST-SUMO-1 protein (38 kDa) and extracts from CAD, HeLa and PC-12 cells, using SUMO-1 Antibody.

SUMO-1 (C9H1) Rabbit mAb - 4940 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C9H1
Western blot analysis of recombinant GST-SUMO-1 protein (38 kDa) and extracts from Jurkat cells using SUMO-1 (C9H1) Rabbit mAb.

SUMO-1 (C21A7) Rabbit mAb - 4931 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone C21A7
Western blot analysis of extracts from CAD, K562, PC-12 and Jurkat cells using SUMO-1 (C21A7) Rabbit mAb.

SUMO-1 Antibody - 4972 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of recombinant GST-SUMO-1 protein (38kD), and extracts from PC12 and CAD cells, using SUMO-1 Antibody.

SUMO-1 (C-19) - sc-6375 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SUMO-1 (C-19): sc-6375. Western blot analysis of human recombinant SUMO-1 fusion protein.

SUMO-1 (D-11) - sc-5308 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SUMO-1 (D-11): sc-5308. Western blot analysis of SUMO-1 expression in HeLa (A), untreated (B) and EGF-treated A-431 (C) whole cell lysates.

SUMO-1 (FL-101) - sc-9060 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SUMO-1 (FL-101): sc-9060. Western blot analysis of SUMO-1 covalently bound to Ran GAP1 in NIH/3T3 (A), KNRK (B) and 3611-RF (C) whole cell lysates.

SUMO-1 (N-19) - sc-6376 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SUMO-1 (N-19): sc-6376. Western blot analysis of human recombinant SUMO-1 fusion protein.

SUMO-1 (S-18) - sc-31852 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SUMO-1 (V-12) - sc-31851 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SUMO-1 (66AT1273.94) - sc-130275 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
SUMO-1 (66AT1273.94): sc-130275. Western blot analysis of SUMO-1 expression in HL60 whole cell lysate.

Anti-Sumo 1 antibody - ab11672 from Abcam

Prices $398.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence of 293T cells transfected with a vector that has Sumo1 fused to GFP and a Flag tag. The top panel is GFP fluorescence. The middle panel uses ab11672 at 1/300 with a rhodamine secondary antibody. The lower panel is a merge of the GFP fluorescence and ab11672 immunostaining. Staining was also seen in untransfected cells (although at a lower level).

Anti-Sumo 1 antibody - ab11291 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab11291 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11291, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Sumo 1 antibody - ab195641 from Abcam

Prices $378.00
Sizes 100 µl
Host Sheep
Clonality Polyclonal
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Sumo 1 with ab195641 at 1/100 dilution.

Anti-Sumo 1 antibody - ab58424 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Sumo 1 antibody (ab58424) at 1/500 dilutionLane 1 : 293 cell extractLane 2 : 293 cell extract with immunizing peptide at 5 µgLysates/proteins at 5 µg per lane.SecondaryAnti-Rabbit IgG

Anti-Sumo 1 antibody - ab22738 from Abcam

Prices $370.00
Sizes 100 µl
Host Sheep
Clonality Polyclonal

Anti-Sumo 1 antibody - ab3819 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Left lane: Western blot (12.5% gel) of sumoylated Topo I protein with Topo I antibody T8N.Right lane: Western blot of sumoylated Topo I protein with ab3819.Both primaries were used a dilution of 1/1000 (60 minutes at 25°C).Topo I protein has multiple sumoylation sites. Four sumoylated Topo I can be seen by Topo I antibody and seven sumoylated Topo I can be seen by Sumo I antibody.

Anti-Sumo 1 antibody [4D12] - BSA and Azide free - ab188281 from Abcam

Prices $384.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone 4D12
Anti-Sumo 1 antibody [4D12] - BSA and Azide free (ab188281) at 1 µg/ml + HeLa total cell extract

Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin) - ab179907 from Abcam

Prices $385.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone 4D12
Immunofluorescent analysis of C-33A Human cervix carcinoma cells labeling Sumo 1 with ab179907 at 10 ug/ml

Anti-Sumo 1 antibody [4D12] - BSA and Azide free (FITC) - ab179893 from Abcam

Prices $378.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone 4D12
Immunofluorescent analysis of C-33A Human cervix carcinoma cells labeling Sumo 1 with ab179893 at 10ug/ml

Anti-Sumo 1 antibody [EP298] - ab133352 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP298
All lanes : Anti-Sumo 1 antibody [EP298] (ab133352) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HL60 cell lysateLane 3 : A431 cell lysateLane 4 : NIH3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Sumo 1 antibody [none] - ab93951 from Abcam

Prices $379.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone none

Anti-Sumo 1 antibody [SM1/495] - ab190759 from Abcam

Prices $379.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone SM1/495

Anti-Sumo 1 antibody [Y299] - ChIP Grade - ab32058 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y299
All lanes : Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : NIH 3T3 cell lysate
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