Anti-Sumo 2+3 antibody [8A2] - ChIP Grade

• Supplier: Abcam
• Catalog: ab81371
• Prices: $401.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone: 8A2
• Applications: WB ICC/IF ICC/IF IP ChIP IHC-P
• Species Reactivities: Human
• Antigen: Recombinant full length Sumo 2 protein (Human)
• Description: Mouse Monoclonal
• Gene: SUMO3
• Conjugate: Unconjugated

Product images

Anti-Sumo 2+3 antibody [8A2] - ChIP Grade (ab81371) at 1/2000 dilution + HeLa cell lysate

Ab81371 (1:2000) staining human Sumo 2 + 3 in HeLa cells by immunofluorescence.

IHC image of ab81371 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81371, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Comprehensive identification of SUMO2/3 targets and their dynamics during - Comprehensive identification of SUMO2/3 targets and their dynamics during

Schou J, Kelstrup CD, Hayward DG, Olsen JV, Nilsson J. PLoS One. 2014 Jun 27;9(6):e100692.

Identification of small ubiquitin-like modifier substrates with diverse functions - Identification of small ubiquitin-like modifier substrates with diverse functions

Ma L, Aslanian A, Sun H, Jin M, Shi Y, Yates JR 3rd, Hunter T. Mol Cell Proteomics. 2014 Jul;13(7):1659-75.

Uncovering global SUMOylation signaling networks in a site-specific manner. - Uncovering global SUMOylation signaling networks in a site-specific manner.

Hendriks IA, D'Souza RC, Yang B, Verlaan-de Vries M, Mann M, Vertegaal AC. Nat Struct Mol Biol. 2014 Oct;21(10):927-36.

SUMOylation of the farnesoid X receptor (FXR) regulates the expression of FXR - SUMOylation of the farnesoid X receptor (FXR) regulates the expression of FXR

Balasubramaniyan N, Luo Y, Sun AQ, Suchy FJ. J Biol Chem. 2013 May 10;288(19):13850-62.

CBX4-mediated SUMO modification regulates BMI1 recruitment at sites of DNA - CBX4-mediated SUMO modification regulates BMI1 recruitment at sites of DNA

Ismail IH, Gagne JP, Caron MC, McDonald D, Xu Z, Masson JY, Poirier GG, Hendzel MJ. Nucleic Acids Res. 2012 Jul;40(12):5497-510.

Small ubiquitin-like modifier (SUMO) modification of zinc finger protein 131 - Small ubiquitin-like modifier (SUMO) modification of zinc finger protein 131

Oh Y, Chung KC. J Biol Chem. 2012 May 18;287(21):17517-29.

Tripartite motif-containing protein 28 is a small ubiquitin-related modifier E3 - Tripartite motif-containing protein 28 is a small ubiquitin-related modifier E3

Liang Q, Deng H, Li X, Wu X, Tang Q, Chang TH, Peng H, Rauscher FJ 3rd, Ozato K, Zhu F. J Immunol. 2011 Nov 1;187(9):4754-63.

Protection from isopeptidase-mediated deconjugation regulates paralog-selective - Protection from isopeptidase-mediated deconjugation regulates paralog-selective

Zhu S, Goeres J, Sixt KM, Bekes M, Zhang XD, Salvesen GS, Matunis MJ. Mol Cell. 2009 Mar 13;33(5):570-80.

SUMO-2/3 modification and binding regulate the association of CENP-E with - SUMO-2/3 modification and binding regulate the association of CENP-E with

Zhang XD, Goeres J, Zhang H, Yen TJ, Porter AC, Matunis MJ. Mol Cell. 2008 Mar 28;29(6):729-41.