All lanes : Anti-Sumo 3 antibody (ab34661) at 1/1000 dilution (5% non-fat dry milk in TTBS)Lane 1 : Human GFP SUMO 3 at 1 µg with blocked for 1 hour with 5% non-fat dry milk in TTBSLane 2 : Human GFP SUMO 3 at 3 µg with blocked for 1 hour with 5% non-fat dry milk in TTBSLane 3 : Human GFP SUMO 3 at 5 µg with blocked for 1 hour with 5% non-fat dry milk in TTBSLane 4 : Human GFP SUMO 3 at 10 µg with blocked for 1 hour with 5% non-fat dry milk in TTBSSecondaryDonkey anti rabbit IgG at 1/1000 dilution
All lanes : Anti-Sumo 3 antibody (ab34661) at 1/1000 dilution (in 5% non-fat dry milk in TTBS)Lane 1 : MW markerLane 2 : GFP human SUMO 3 at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBSLane 3 : GFP human SUMO 1 at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBSLane 4 : GFP yeast SUMO at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBSLane 5 : GFP Arabidopsis thaliana SUMO 1 at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBSLane 6 : GFP Arabidopsis thaliana SUMO 2 at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBSLane 7 : GFP tomato SUMO at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBSSecondaryDonkey anti-rabbit IgG at 1/1000 dilution