Anti-SUMO4 antibody [EPR7163]

• Supplier: Abcam
• Catalog: ab126606
• Prices: $313.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR7163
• Applications: WB IP FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide, corresponding to residues in Human SUMO4
• Description: Rabbit Monoclonal
• Gene: SUMO4
• Conjugate: Unconjugated

Product images

Overlay histogram showing HEK293 cells stained with ab126606 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126606, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

ab126606 at 1/250 dilution staining SUMO4 in HepG2 cells by immunofluorescence.

All lanes : Anti-SUMO4 antibody [EPR7163] (ab126606) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLane 4 : Human spleen tissue lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD