Anti-SUN2 antibody [EPR6557]

• Supplier: Abcam
• Catalog: ab124916
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR6557
• Applications: WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to C terminal residues of Human SUN2
• Description: Rabbit Monoclonal
• Gene: SUN2
• Conjugate: Unconjugated

Product images

ab124916 staining SUN2 in mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An HRP-conjugated goat anti-rabbit IgG, ab97051 (1/500) was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

ab124916 staining SUN2 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An  HRP-conjugated Goat anti-rabbit IgG, ab97051 (1/500), was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

Anti-SUN2 antibody [EPR6557] (ab124916) at 1/5000 dilution + Rat brain lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Anti-SUN2 antibody [EPR6557] (ab124916) at 1/5000 dilution + Mouse heart lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

All lanes : Anti-SUN2 antibody [EPR6557] (ab124916) at 1/5000 dilutionLane 1 : HeLa cell LysateLane 2 : Jurkat cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

All lanes : Anti-SUN2 antibody [EPR6557] (ab124916) at 1/1000 dilution (unpurified)Lane 1 : Human fetal muscle lysateLane 2 : Saos-2 lysateLane 3 : HeLa lysateLane 4 : Jurkat lysateLane 5 : HepG2 lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

ab124916, unpurified, at a 1/250 dilution, staining SUN2 in paraffin embedded Human lung tissue by Immunohistochemistry.

ab124916, unpurified, at a 1/250 dilution, staining SUN2 in paraffin embedded Human ovarian tissue by Immunohistochemistry.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD