Anti-Suppressor of Fused antibody [EP1207Y]
| Name | Anti-Suppressor of Fused antibody [EP1207Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab52913 |
| Prices | $397.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1207Y |
| Applications | WB IP ICC/IF FC IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to a region of aa 355 to 380 of human Suppressor of Fused |
| Description | Rabbit Monoclonal |
| Gene | SUFU |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Suppressor of Fused antibody [EP1207Y] (ab52913) at 1/200 dilution + LnCaP cell lysate at 10 µgSecondaryGoat anti rabbit HRP antibody at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/6960_ab52913wb.jpg)
Anti-Suppressor of Fused antibody [EP1207Y] (ab52913) at 1/200 dilution + LnCaP cell lysate at 10 µgSecondaryGoat anti rabbit HRP antibody at 1/2000 dilution
Ab52913 at 1/100 dilution staining human fetal kidney tissue; paraffin embedded.
Overlay histogram showing HEK293 cells stained with ab52913 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52913, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
RIO kinase 3 acts as a SUFU-dependent positive regulator of Hedgehog signaling. - RIO kinase 3 acts as a SUFU-dependent positive regulator of Hedgehog signaling.
Tariki M, Wieczorek SA, Schneider P, Banfer S, Veitinger S, Jacob R, Fendrich V, Lauth M. Cell Signal. 2013 Dec;25(12):2668-75.
Kif7 promotes hedgehog signaling in growth plate chondrocytes by restricting the - Kif7 promotes hedgehog signaling in growth plate chondrocytes by restricting the
Hsu SH, Zhang X, Yu C, Li ZJ, Wunder JS, Hui CC, Alman BA. Development. 2011 Sep;138(17):3791-801.