ab17781 staining Surfactant protein D in human gall bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/500 in PBS) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. Counterstained with DAPI.See Abreview
ab17781 staining Surfactant protein D in human gall bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% serum for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/400 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated horse anti-mouse IgG polyclonal was used as the secondary antibody.See Abreview
ab17781 at a 1/500 dilution staining perinatal mouse lung tissue sections by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and blocked with 2.5% BSA for 30 minutes. The antibody was incubated with the tissue for 16 hours and bound antibody was detected with an Alexa-Fluor ® 594 conjugated Goat anti-Mouse IgG antibody.This image is courtesy of an Anonymous Abreview submitted on 3 April 2006.See Abreview