Anti-Survivin antibody
| Name | Anti-Survivin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab469 |
| Prices | $403.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P ELISA FC IP Radioimmunoprecipitation |
| Species Reactivities | Mouse, Rat, Bovine, Cat, Dog, Human |
| Antigen | Recombinant full length protein corresponding to Human Survivin |
| Description | Rabbit Polyclonal |
| Gene | BIRC5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab469 staining Survivin from Ovarian carcionoma tumour tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed (Citrate buffer pH=6, microwave oven) and the tissue was then formaldehyde fixed and blocked (Hydrogen peroxide 0.03%). An HRP conjugated goat anti-rabbit was used as the secondary antibody.See Abreview
HeLa cells (ab150035) in prometaphase, metaphase and anaphase stained with anti-Survivin (green), anti-tubulin (red) and DAPI (blue). These images were kindly supplied as part of the review submitted by William Moore, University of Dundee, UK.
ab469 at a 1/400 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then was detected using a Texas Red conjugated Goat anti-rabbit antibody. This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 30 March 2006.See Abreview
All lanes : Anti-Survivin antibody (ab469) at 1 µg/mlLane 1 : HeLa NuclearLane 2 : HeLa whole cell lysateLane 3 : A431 cell lysateLane 4 : Jurkat cell lysateLane 5 : HEK293 cell lysateLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor anti-rabbit at 1/5000 dilutionPerformed under reducing conditions.
ab469 immunoprecipitating Survivin from HeLa cell lysate. HeLa cells were lysed after colcemid block (ON) and immunoprecipitated with ab469 at a 1/1000 dilution. HeLa cells clear lysate (Lane 1) as well as the bound material (Lane 2) were loaded on a 15 % acrylamide gel. An HRP conjugated Donkey Anti-rabbit IgG was used as the secondary antibody. This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 12 April 2006.See Abreview
Product References
Expression of nuclear survivin in normal skin and squamous cell carcinoma: a - Expression of nuclear survivin in normal skin and squamous cell carcinoma: a
Dallaglio K, Petrachi T, Marconi A, Truzzi F, Lotti R, Saltari A, Morandi P, Puviani M, Maiorana A, Pincelli C. Br J Cancer. 2014 Jan 7;110(1):199-207.
Clinical significance of survivin expression in patients with urothelial - Clinical significance of survivin expression in patients with urothelial
Chen HA, Su CM, Hsieh HY, Tung CL, Hsu CD, Wang YH, Shen CH. Dis Markers. 2014;2014:574985.
BRCA1-IRIS overexpression promotes formation of aggressive breast cancers. - BRCA1-IRIS overexpression promotes formation of aggressive breast cancers.
Shimizu Y, Luk H, Horio D, Miron P, Griswold M, Iglehart D, Hernandez B, Killeen J, ElShamy WM. PLoS One. 2012;7(4):e34102.
Differential transcriptomic analysis of spontaneous lung tumors in B6C3F1 mice: - Differential transcriptomic analysis of spontaneous lung tumors in B6C3F1 mice:
Pandiri AR, Sills RC, Ziglioli V, Ton TV, Hong HH, Lahousse SA, Gerrish KE, Auerbach SS, Shockley KR, Bushel PR, Peddada SD, Hoenerhoff MJ. Toxicol Pathol. 2012 Dec;40(8):1141-59.
Combined inhibition of DNA methyltransferase and histone deacetylase restores - Combined inhibition of DNA methyltransferase and histone deacetylase restores
Kaminskyy VO, Surova OV, Vaculova A, Zhivotovsky B. Carcinogenesis. 2011 Oct;32(10):1450-8.
Acetonic extract of Buxus sempervirens induces cell cycle arrest, apoptosis and - Acetonic extract of Buxus sempervirens induces cell cycle arrest, apoptosis and
Ait-Mohamed O, Battisti V, Joliot V, Fritsch L, Pontis J, Medjkane S, Redeuilh C, Lamouri A, Fahy C, Rholam M, Atmani D, Ait-Si-Ali S. PLoS One. 2011;6(9):e24537.
Endogenous survivin modulates survival and proliferation in UVB-treated human - Endogenous survivin modulates survival and proliferation in UVB-treated human
Dallaglio K, Palazzo E, Marconi A, Dumas M, Truzzi F, Lotti R, Bonte F, Pincelli C. Exp Dermatol. 2009 May;18(5):464-71.
Non-coding murine centromeric transcripts associate with and potentiate Aurora B - Non-coding murine centromeric transcripts associate with and potentiate Aurora B
Ferri F, Bouzinba-Segard H, Velasco G, Hube F, Francastel C. Nucleic Acids Res. 2009 Aug;37(15):5071-80.
Hyaluronan-CD44 interaction with protein kinase C(epsilon) promotes oncogenic - Hyaluronan-CD44 interaction with protein kinase C(epsilon) promotes oncogenic
Bourguignon LY, Spevak CC, Wong G, Xia W, Gilad E. J Biol Chem. 2009 Sep 25;284(39):26533-46.
The Nup107-160 nucleoporin complex promotes mitotic events via control of the - The Nup107-160 nucleoporin complex promotes mitotic events via control of the
Platani M, Santarella-Mellwig R, Posch M, Walczak R, Swedlow JR, Mattaj IW. Mol Biol Cell. 2009 Dec;20(24):5260-75.