Anti-SUZ12 antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab12073
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IP ChIP ChIP WB ChIP Radioimmunoprecipitation IHC-P
• Species Reactivities: Mouse, Human, Marmoset
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Human SUZ12
• Description: Rabbit Polyclonal
• Gene: SUZ12
• Conjugate: Unconjugated

Product images

All lanes : Anti-SUZ12 antibody - ChIP Grade (ab12073) at 0.4 µg/mlLane 1 : Untransfected 293T cellsLane 2 : 293T cells transfected with 5ug HA-SUZ12developed using the ECL technique

All lanes : Anti-SUZ12 antibody - ChIP Grade (ab12073) at 1 µg/mlLane 1 : untransfected 293T cell lysateLane 2 : 293T cells transfected with 5ug HA-Suz12Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG H&L (HRP) (Dako) at 1/2000 dilutionPerformed under reducing conditions.

All lanes : Anti-SUZ12 antibody - ChIP Grade (ab12073) at 1 µg/mlLane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Chromatin was prepared from the Human Foreskin Fibroblasts cells. The cross-linking (X-ChiP) technique was used, crosslinking was done for 9 minutes in serum free 1% formaldehyde reagent. The primary antibody was diluted 1/500 in phosphate buffered ChiP dilution buffer and incubated with the sample for 16 hours at 4°C. HOX C13 region was used on the positive control, whilst normal rabbit IgG and GAPDH was used in the negative control. The immunoprecipitated DNA was quantified by real time PCR.See Abreview

Chromatin was prepared from nuclear lysate of the human MDA-MB-231 breast epithelial adenocarcinoma cells. The cross-linking (X-ChiP) technique was used, crosslinking was performed for 15 minutes in formaldehyde. The primary antibody was used in concentration of 0.2 µg/µg chromatin and incubated with the sample for 16 hours at 4°C in SDS, DOC, TritonX-100, EDTA, HEPES, NaCl. The immunoprecipitated DNA was quantified by real time PCR. Ct values were converted to DNA copy numbers using a standard curve in the Q-PCR step. The number of binding events detected for each test reaction was then calculated by taking into account the DNA copy number, cell equivalents of chromatin used in the ChIP and PCR, and primer pair amplification efficiency.See Abreview

IHC-P image of Suz12 staining with ab12073 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab12073 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.See Abreview

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