SUZ12

Gene Symbol SUZ12
Entrez Gene 23512
Alt Symbol CHET9, JJAZ1
Species Human
Gene Type protein-coding
Description SUZ12 polycomb repressive complex 2 subunit
Other Description chET 9 protein|chromatin precipitated E2F target 9 protein|joined to JAZF1 protein|polycomb protein SUZ12|suppressor of zeste 12 homolog|suppressor of zeste 12 protein homolog
Swissprots Q96BD9 Q15022
Accessions EAW80258 Q15022 AK074333 AK290014 BAF82703 BC015704 AAH15704 BC018583 D63881 BAA09931 DB501687 ES313164 XM_005257954 XP_005258011 XM_006721794 XP_006721857 XM_011524576 XP_011522878 XM_011524577 XP_011522879 XM_011524578 XP_011522880 NM_015355 NP_056170
Function Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. {ECO:0000269|PubMed:15225548, ECO:0000269|PubMed:15231737, ECO:0000269|PubMed:15385962, ECO:0000269|PubMed:16618801, ECO:0000269|PubMed:17344414, ECO:0000269|PubMed:18285464}.
Subcellular Location Nucleus {ECO:0000269|PubMed:15231737}.
Tissue Specificity Overexpressed in breast and colon cancer. {ECO:0000269|PubMed:15231737, ECO:0000269|PubMed:15684044}.

SUZ12 (D39F6) XP ® Rabbit mAb (Biotinylated) - 13701 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D39F6
Western blot analysis of extracts from C6 and HeLa cells using SUZ12 (D39F6) XP ® Rabbit mAb (Biotinylated).

SUZ12 (D39F6) XP ® Rabbit mAb - 3737 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D39F6
Western blot analysis of extracts from various cell lines using SUZ12 (D39F6) XP ® Rabbit mAb.

SUZ12 (P-15) - sc-46264 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SUZ12 (P-15)): sc-46264. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear and cytoplasmic localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

SUZ12 (D-10) - sc-271325 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SUZ12 (D-10): sc-271325. Western blot analysis of SUZ12 expression in HeLa whole cell lysate.

SUZ12 (G-18) - sc-46263 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SUZ12 (H-300) - sc-67105 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SUZ12 (H-300): sc-67105. Western blot analysis of SUZ12 expression in non-transfected 293: sc-110760 (A), human SUZ12 transfected 293: sc-111839 (B), HeLa (C), SW480 (D) and JEG-3 (E) whole cell lysates.

Anti-SUZ12 antibody - ab107249 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
All lanes : Anti-SUZ12 antibody (ab107249)Lane 1 : HEK293 cell lysateLane 2 : SUZ12-hIgGFc transfected HEK293 cell lysate

Anti-SUZ12 antibody - ab191573 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-SUZ12 antibody (ab191573) at 1 µg/ml + Human liver tissue lysate at 15 µg

Anti-SUZ12 antibody - ab77605 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-SUZ12 antibody (ab77605) at 1 µg/ml + Human SUZ12 Partial Recombinant Protein (His Tagged) at 0.1 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-SUZ12 antibody [2C11] - ab107253 from Abcam

Host Mouse
Clonality Monoclonal
Clone 2C11
Immunofluorescence analysis of U251 cells using 1/200 ab107253 (green). Actin filaments labelled with Alexa Fluor-555 phalloidin in red.

Anti-SUZ12 antibody [EPR5234(N)] - ab175187 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5234(N)
All lanes : Anti-SUZ12 antibody [EPR5234(N)] (ab175187) at 1/1000 dilutionLane 1 : SW480 cell lysatesLane 2 : HeLa cell lysatesLane 3 : MCF-7 cell lysatesLane 4 : 293T cell lysatesLysates/proteins at 10 µg per lane.

Anti-SUZ12 antibody [EPR5234(N)] (Alexa Fluor® 647) - ab196975 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5234(N)
ab196975 staining SUX12 in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196975 at a 1/500 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in Hek293 cells fixed with 4% formaldehyde (10 min).

Anti-SUZ12 antibody [EPR5234(N)] (HRP) - ab196976 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5234(N)
All lanes : Anti-SUZ12 antibody [EPR5234(N)] (HRP) (ab196976) at 1/3000 dilutionLane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-SUZ12 antibody [SUZ220A] - ab126577 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone SUZ220A
Overlay histogram showing MCF7 cells stained with ab126577 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126577, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-SUZ12 antibody - ChIP Grade - ab12073 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SUZ12 antibody - ChIP Grade (ab12073) at 0.4 µg/mlLane 1 : Untransfected 293T cellsLane 2 : 293T cells transfected with 5ug HA-SUZ12developed using the ECL technique

Anti-SUZ12 antibody - N-terminal - ab135097 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-SUZ12 antibody - N-terminal (ab135097) at 1/1000 dilution + Hela total cell extract

Anti-SUZ12 Antibody (aa362-411) IHC-plus™ - LS-B5591 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-SUZ12 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5591 concentration 5 ug/ml.

Anti-SUZ12 Antibody (clone 3D10) IHC-plus™ - LS-B10727 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 3D10
Anti-SUZ12 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10727 dilution 1:100.

Anti-SUZ12 Antibody (aa131-305) - LS-C76167 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-SUZ12 Antibody - LS-C39346 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-SUZ12 Antibody (aa559-588) - LS-C203831 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
SUZ12 Antibody western blot of Ramos cell line lysates (35 ug/lane). The SUZ12 antibody detected the SUZ12 protein (arrow).

Anti-SUZ12 Antibody (aa689-739) - LS-C289153 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SUZ12 Antibody (C-Terminus) - LS-C80253 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-SUZ12 Antibody (N-Terminus) - LS-C347706 from LifeSpan Bioscience

Prices $405.00
Sizes 25 µg
Host Rabbit
Clonality Polyclonal

Anti-SUZ12 Antibody (clone 2C11) - LS-C169292 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2C11
Immunofluorescence of MCF-7 cells using SUZ12 mouse monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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