Anti-SV2A antibody
| Name | Anti-SV2A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab32942 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IHC-P WB ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Rat, Human, Bovine |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat SV2A |
| Description | Rabbit Polyclonal |
| Gene | SV2A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : MarkerLanes 2 - 4 : Anti-SV2A antibody (ab32942) at 1/500 dilutionLane 1 : As aboveLane 2 : Mouse Brain Tissue Lysate at 20 µgLane 3 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 20 µgLane 4 : Rat Brain Tissue Lysate at 20 µgSecondaryLanes 2 - 4 : IR Dye 680 Conjugated Goat anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
![SV2A was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to SV2A and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32942.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 82kDa: SV2A](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/7198_SV2A-Primary-antibodies-ab32942-11.jpg)
SV2A was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to SV2A and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32942.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 82kDa: SV2A
Immunostaining for SV2A in the rat hippocampus obtained with the antibody ab32942 (1:1000), using direct fluorescence. Please see accompanying abreview for additional information.See Abreview
Product References
Antiepileptic effects of levetiracetam in a rodent neonatal seizure model. - Antiepileptic effects of levetiracetam in a rodent neonatal seizure model.
Talos DM, Chang M, Kosaras B, Fitzgerald E, Murphy A, Folkerth RD, Jensen FE. Pediatr Res. 2013 Jan;73(1):24-30.
Effect of delayed peripheral nerve repair on nerve regeneration, Schwann cell - Effect of delayed peripheral nerve repair on nerve regeneration, Schwann cell
Jonsson S, Wiberg R, McGrath AM, Novikov LN, Wiberg M, Novikova LN, Kingham PJ. PLoS One. 2013;8(2):e56484.
Expression of SV2 isoforms during rodent brain development. - Expression of SV2 isoforms during rodent brain development.
Crevecoeur J, Foerch P, Doupagne M, Thielen C, Vandenplas C, Moonen G, Deprez M, Rogister B. BMC Neurosci. 2013 Aug 9;14:87.