Anti-Synaptophysin antibody
| Name | Anti-Synaptophysin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53166 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide derived from human Synaptophysin |
| Description | Rabbit Polyclonal |
| Gene | SYP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Synaptophysin antibody (ab53166) at 1/500 dilutionLane 1 : LOVO cell extractLane 2 : LOVO cell extract with immunising peptide
ab53166 at 1/50 dilution staining Synaptophysin in Human brain by Immunohistochemsitry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
ICC/IF image of ab53166 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53166 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Bate C, Tayebi M, Williams A. Mol Neurodegener. 2010 Apr 8;5:13.
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Castellino RC, Barwick BG, Schniederjan M, Buss MC, Becher O, Hambardzumyan D, Macdonald TJ, Brat DJ, Durden DL. PLoS One. 2010 May 26;5(5):e10849.