Anti-Synaptophysin antibody [EP1098Y]

• Supplier: Abcam
• Catalog: ab52636
• Prices: $403.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1098Y
• Applications: IHC-P WB IP FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues on Synaptophysin (Human)
• Description: Rabbit Monoclonal
• Gene: SYP
• Conjugate: Unconjugated

Product images

Anti-Synaptophysin antibody [EP1098Y] (ab52636) at 1/200000 dilution + Rat brain membraneSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution

Human medulloblastoma labelled with ab52636 at 1/250 - 1/500 dilution

PC12 cells labelled with ab52636 at 1/250 - 1/500 dilution

Overlay histogram showing PC12 cells stained with ab52636 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52636, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Product References

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