Anti-Synphilin 1 antibody
| Name | Anti-Synphilin 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab6179 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IHC-F WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide: CASKGKNKAA conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 911-919 of Human Synphilin 1 |
| Description | Rabbit Polyclonal |
| Gene | SNCAIP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab6179 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab6179 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab6179 staining in rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6179, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Parkin expression in the developing mouse. - Parkin expression in the developing mouse.
Kuhn K, Zhu XR, Lubbert H, Stichel CC. Brain Res Dev Brain Res. 2004 Apr 19;149(2):131-42.
Deletion of the alpha-synuclein locus in a subpopulation of C57BL/6J inbred mice. - Deletion of the alpha-synuclein locus in a subpopulation of C57BL/6J inbred mice.
Specht CG, Schoepfer R. BMC Neurosci. 2001;2:11. Epub 2001 Aug 24.