Anti-Syntenin antibody [EPR8102]

• Supplier: Abcam
• Catalog: ab133267
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR8102
• Applications: WB IP IHC-P ICC/IF FC
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to a region within Human Syntenin (UniProt O00560)
• Description: Rabbit Monoclonal
• Gene: SDCBP
• Conjugate: Unconjugated

Product images

Overlay histogram showing SHSY-5Y cells stained with ab133267 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133267, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-Syntenin antibody [EPR8102] (ab133267) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : 293T cell lysateLane 3 : Human fetal heart lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Immunohistochemical analysis of Syntenin in paraffin embedded Human brain tissue, using ab133267 at a 1/50 dilution.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD