All lanes : Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873) at 1/1000 dilutionLane 1 : HT29 lysateLane 2 : HepG2 lysateLane 3 : A549 lysateLane 4 : MCF7 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873) at 1/1000 dilution + Human muscle lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873) at 1/1000 dilution + NIH 3T3 lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Syntrophin alpha 1 with ab188873 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling Syntrophin alpha 1 with ab188873 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Flow cytometric analysis of 2% paraformaldehyde-fixed HT29 cells labeling Syntrophin alpha 1 with ab188873 at 1/280 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling Syntrophin alpha 1 with ab188873 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).