Anti-T-bet / Tbx21 antibody [4B10]
| Name | Anti-T-bet / Tbx21 antibody [4B10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab91109 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 4B10 |
| Applications | IHC-P WB IP FC |
| Species Reactivities | Mouse, Human |
| Antigen | E |
| Description | Mouse Monoclonal |
| Gene | TBX21 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-T-bet / Tbx21 antibody [4B10] (ab91109) at 2 µg/mlLane 1 : Lysates of CD4+ T cells (control)Lane 2 : Lysates of PMA and Ionomycin reactivated CD4+ T cellsSecondaryHRP anti-mouse IgG](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/7926_T-bet-Tbx21-Primary-antibodies-ab91109-2.JPG)
All lanes : Anti-T-bet / Tbx21 antibody [4B10] (ab91109) at 2 µg/mlLane 1 : Lysates of CD4+ T cells (control)Lane 2 : Lysates of PMA and Ionomycin reactivated CD4+ T cellsSecondaryHRP anti-mouse IgG
![Overlay histogram showing Jurkat cells stained with ab91109 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91109, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/7927_T-bet-Tbx21-Primary-antibodies-ab91109-6.jpg)
Overlay histogram showing Jurkat cells stained with ab91109 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91109, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
IHC image of T-bet / Tbx21 staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91109, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
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Adiponectin induces pro-inflammatory programs in human macrophages and CD4+ T - Adiponectin induces pro-inflammatory programs in human macrophages and CD4+ T
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A novel transcription factor, T-bet, directs Th1 lineage commitment. - A novel transcription factor, T-bet, directs Th1 lineage commitment.
Szabo SJ, Kim ST, Costa GL, Zhang X, Fathman CG, Glimcher LH. Cell. 2000 Mar 17;100(6):655-69.