Anti-Talin 1 antibody
| Name | Anti-Talin 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57758 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG3 |
| Applications | WB IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment: SALSVVQNLE KDLQEVKAAA RDGKLKPLPG ETMEKCTQDL GNSTKAVSSA IAQLLGEVAQ GNENYAGIAA RDVAGGLRSL AQAARGVAAL TSDPAVQA, corresponding to amino acids 1052-1150 of Human Talin Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | TLN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Talin antibody (ab57758) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
ICC/IF image of ab57758 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57758, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing RAW 264.7 cells stained with ab57758 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57758, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in RAW 264.7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/8317_Talin-1-Primary-antibodies-ab57758-2.jpg)
Overlay histogram showing RAW 264.7 cells stained with ab57758 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57758, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in RAW 264.7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Fine tuning inflammation at the front door: macrophage complement receptor - Fine tuning inflammation at the front door: macrophage complement receptor
Dai S, Rajaram MV, Curry HM, Leander R, Schlesinger LS. PLoS Pathog. 2013 Jan;9(1):e1003114.
Kindlins, integrin activation and the regulation of talin recruitment to - Kindlins, integrin activation and the regulation of talin recruitment to
Kahner BN, Kato H, Banno A, Ginsberg MH, Shattil SJ, Ye F. PLoS One. 2012;7(3):e34056.
Altered expression of talin 1 in peripheral immune cells points to a significant - Altered expression of talin 1 in peripheral immune cells points to a significant
Degroote RL, Hauck SM, Kremmer E, Amann B, Ueffing M, Deeg CA. J Proteomics. 2012 Jul 19;75(14):4536-44.