All lanes : Anti-TAOK1 antibody [EPR12908(2)] (ab197891) at 1/1000 dilutionLane 1 : Human seminoma lysateLane 2 : Human testis lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-TAOK1 antibody [EPR12908(2)] (ab197891) at 1/10000 dilutionLane 1 : HeLa whole (Human epithelial cells from cervix adenocarcinoma) cell lysateLane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-TAOK1 antibody [EPR12908(2)] (ab197891) at 1/1000 dilutionLane 1 : Rat brain lysateLane 2 : Rat heart lysateLane 3 : Rat spleen lysateLane 4 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 5 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution