Anti-TBC1D4 (phospho T642) antibody

• Supplier: Abcam
• Catalog: ab65753
• Prices: $378.00
• Sizes: 100 µg
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide: CRRRAHTPFSHPP conjugated to KLH, corresponding to amino acids 637-647 of Human TBC1D4 Run BLAST with Run BLAST with
• Description: Sheep Polyclonal
• Gene: TBC1D4
• Conjugate: Unconjugated

Product images

All lanes : Anti-TBC1D4 (phospho T642) antibody (ab65753) at 2 µg/mlLane 1 : AS160 wild type (WT)Lane 2 : AS160 wild type (WT) with (+) 1 µM insulin treatment for 15 minutes Lane 3 : S588A mutant (S588A)Lane 4 : S588A mutant (S588A) with (+) 1 µM insulin treatment for 15 minutes Lane 5 : T642A mutant (T642A) Lane 6 : T642A mutant (T642A) with (+) 1 µM insulin treatment for 15 minutes

ICC/IF image of ab65753 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65753, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96939, DyLight® 488 donkey anti-sheep IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Muscle-specific knock-out of NUAK family SNF1-like kinase 1 (NUAK1) prevents high - Muscle-specific knock-out of NUAK family SNF1-like kinase 1 (NUAK1) prevents high

Inazuka F, Sugiyama N, Tomita M, Abe T, Shioi G, Esumi H. J Biol Chem. 2012 May 11;287(20):16379-89.

Sustained postexercise increases in AS160 Thr642 and Ser588 phosphorylation in - Sustained postexercise increases in AS160 Thr642 and Ser588 phosphorylation in

Schweitzer GG, Arias EB, Cartee GD. J Appl Physiol (1985). 2012 Dec 15;113(12):1852-61. doi: