TBC1D4

Gene Symbol TBC1D4
Entrez Gene 9882
Alt Symbol AS160, NIDDM5
Species Human
Gene Type protein-coding
Description TBC1 domain family, member 4
Other Description Acrg embryonic lethality minimal region ortholog|TBC (Tre-2, BUB2, CDC16) domain-containing protein|TBC1 domain family member 4|akt substrate of 160 kDa
Swissprots A7E2X8 B6ETN8 B6ETN9 Q68D14 O60343 Q5W0B9 B4DU25 B4E235
Accessions EAW80536 EAW80537 EAW80538 O60343 AB011175 BAA25529 AB449885 BAH16628 AK300468 BAG62187 AK304091 BAG64997 AK307493 AY211910 BC146300 AAI46301 BC151239 AAI51240 CA448893 CR749622 CAH18416 FM207106 CAR62509 FM207107 CAR62510 XM_005266603 XP_005266660 XM_005266605 XP_005266662 XM_006719903 XP_006719966 XM_011535331 XP_011533633 NM_001286658 NP_001273587 NM_001286659 NP_001273588 NM_014832 NP_055647
Function May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. {ECO:0000269|PubMed:15971998, ECO:0000269|PubMed:18771725, ECO:0000269|PubMed:22908308}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:18771725}. Note=Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
Tissue Specificity Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis. {ECO:0000269|PubMed:15304337, ECO:0000269|PubMed:18276765, ECO:0000269|PubMed:18771725}.

Phospho-AS160 (Ser588) (D8E4) Rabbit mAb - 8730 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8E4
Western blot analysis of extracts from serum-starved HeLa cells, untreated or treated with hIGF-I #8917 (100 ng/ml, 15 min), using Phospho-AS160 (Ser588) (D8E4) Rabbit mAb (upper) or AS160 (C69A7) Rabbit mAb #2670 (lower). The phospho-specificity of the antibody is verified by λ phosphatase treatment.

Phospho-AS160 (Thr642) Antibody - 4288 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from serum starved HeLa cells, untreated or treated with either insulin (150 nM, 15 minutes) or IGF-1 #3093 (100 ng/ml, 15 minutes), using Phospho-AS160 (Thr642) Antibody (upper) or AS160 (C69A7) Rabbit mAb #2670 (lower). The phospho-specificity of the antibody is verified by λ-phosphatase treatment of the above cell lysates.

Phospho-AS160 (Thr642) (D27E6) Rabbit mAb - 8881 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D27E6
Western blot analysis of extracts from serum starved HeLa cells, untreated (-) or insulin-treated (150 nM, 15 min), using Phospho-AS160 (Thr642) (D27E6) Rabbit mAb (upper) or AS160 (C69A7) Rabbit mAb #2670 (lower). The phospho-specificity of the antibody is verified by λ phosphatase treatment.

Phospho-AS160 (Ser318) (D3D11) Rabbit mAb - 8619 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3D11
Western blot analysis of extracts from serum-starved HeLa cells, untreated or treated with either insulin (150 nM, 15 min) or hIGF-I #8917 (100 ng/ml, 15 min), using Phospho-AS160 (Ser318) (D3D11) Rabbit mAb (upper) or AS160 (C69A7) Rabbit mAb #2670 (lower). The phospho-specificity of the antibody is verified by λ phosphatase treatment.

AS160 (C69A7) Rabbit mAb - 2670 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C69A7
Western blot analysis of extracts from HepG2, RD and 293T cells using AS160 (C69A7) Rabbit mAb.

AS160 Antibody - 2447 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HepG2 and 293 cells using AS160 Antibody.

TBC1D4 (H-48) - sc-135256 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

TBC1D4 (N-17) - sc-47470 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TBC1D4 (N-17): sc-47470. Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic staining of glandular cells.

Anti-TBC1D4 (phospho S588) antibody - ab65754 from Abcam

Prices $370.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal

Anti-TBC1D4 (phospho T642) antibody - ab65753 from Abcam

Prices $378.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal
All lanes : Anti-TBC1D4 (phospho T642) antibody (ab65753) at 2 µg/mlLane 1 : AS160 wild type (WT)Lane 2 : AS160 wild type (WT) with (+) 1 µM insulin treatment for 15 minutes Lane 3 : S588A mutant (S588A)Lane 4 : S588A mutant (S588A) with (+) 1 µM insulin treatment for 15 minutes Lane 5 : T642A mutant (T642A) Lane 6 : T642A mutant (T642A) with (+) 1 µM insulin treatment for 15 minutes

Anti-TBC1D4 (phospho T642) antibody - ab59173 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue labelled with ab59173 at 1/50-1/100 dilution. Samples were treated with(+) or without(-) peptide.

Anti-TBC1D4 (phospho T642) antibody [EPR2733(2)] - ab131214 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2733(2)
All lanes : Anti-TBC1D4 (phospho T642) antibody [EPR2733(2)] (ab131214) at 1/1000 dilutionLane 1 : 293T cell lysate - untreatedLane 2 : 293T cell lysate - insulin-treated

Anti-TBC1D4 antibody - ab24469 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TBC1D4 antibody (ab24469) at 0.46 µg/mlLane 1 : Human skeletal muscle cell lysate at 10 µgLane 2 : Human skeletal muscle cell lysate at 20 µgLane 3 : Human skeletal muscle cell lysate at 40 µgLane 4 : Human skeletal muscle cell lysate at 60 µgSecondaryAnti-rabbit HRP

Anti-TBC1D4 antibody - ab62487 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence image of ab 62487 (1/1000 dilution) stained HeLa cells with (1) and without (2) peptide

Anti-TBC1D4 antibody - ab115893 from Abcam

Prices $378.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab115893, at 3.75 ug/ml, staining TBC1D4 in Formalin-fixed, Paraffin-embedded Human skeletal muscle tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-TBC1D4 antibody - ab134744 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TBC1D4 antibody (ab134744) at 0.1 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from 293T cells at 50 µgLane 4 : Whole cell lysate from Jurkat cells at 50 µgdeveloped using the ECL technique

Anti-TBC1D4 antibody - ab134749 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TBC1D4 antibody (ab134749) at 0.1 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from 293T cells at 50 µgLane 4 : Whole cell lysate from Jurkat cells at 50 µgdeveloped using the ECL technique

Anti-TBC1D4 antibody - ab59121 from Abcam

Prices $378.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-TBC1D4 antibody (ab59121) at 0.1 µg/ml (incubated for 1 hour) + Daudi cell lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-TBC1D4 antibody - ab91242 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-TBC1D4 antibody (ab91242) at 1/100 dilution + MDA-MB231 cell line lysates at 35 µg

Anti-TBC1D4 antibody [5E6] - ab119004 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5E6
All lanes : Anti-TBC1D4 antibody [5E6] (ab119004) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs and lysedLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY TBC1D4 cDNA for 48 hrs and lysed Lysates/proteins at 5 µg per lane.

Anti-TBC1D4 / AS160 Antibody (phospho-Thr642) - LS-C34921 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-TBC1D4 / AS160 Antibody (aa1178-1189) - LS-C63888 from LifeSpan Bioscience

Prices $455.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TBC1D4 / AS160 Antibody - LS-C126663 from LifeSpan Bioscience

Prices $355.00
Sizes 40 µl
Host Rabbit
Clonality Polyclonal

Anti-TBC1D4 / AS160 Antibody - LS-C147066 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TBC1D4 / AS160 Antibody (phospho-Ser666) - LS-C125034 from LifeSpan Bioscience

Prices $445.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal
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