All lanes : Anti-TBC1D4 antibody [5E6] (ab119004) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs and lysedLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY TBC1D4 cDNA for 48 hrs and lysed Lysates/proteins at 5 µg per lane.
ab119004, at a 1/100 dilution, staining TBC1D4 in COS7 cells transiently transfected with pCMV6-ENTRY TBC1D4, by Immunofluorescence.
ab119004 at 1/100 dilution staining TBC1D4 in HEK293T cells transfected with either pCMV6-ENTRY TBC1D4 overexpress plasmid (Red) or empty vector control plasmid(Blue) by Flow Cytometry.
ab119004 at 1/100 dilution staining TBC1D4 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
ab119004 at 1/100 dilution staining TBC1D4 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).