All lanes : Anti-Tbx6 antibody (ab38883) at 1 µg/mlLane 1 : Testis (Mouse) Tissue LysateLane 2 : Lung (Mouse) Tissue LysateLane 3 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 4 : Lung (Human) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
IHC-P image of Tbx6 staining with ab38883 on tissue sections from an E8.5 mouse embryo. The sections were Paraformaldehyde fixed, and permeabilized in PBST (PBS/0.5% v/v TritonX100), before incubation with ab38883 (1/500 dilution) for 48 hours at 4°C in 1% BSA, 5% donkey serum in PBST. The secondary used was an Alexa-Fluor 555 conjugated donkey anti-rabbit polyclonal, at a 1/1000 dilution.See Abreview