Anti-Tcl1 antibody [EPR3949]

• Supplier: Abcam
• Catalog: ab108978
• Prices: $99.00, $357.00
• Sizes: 10 µl, 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3949
• Applications: IHC-F WB IHC-P ICC/IF FC
• Species Reactivities: Human
• Antigen: A synthetic peptide corresponding to residues in Human Tcl1
• Description: Rabbit Monoclonal
• Gene: TCL1A
• Conjugate: Unconjugated

Product images

Overlay histogram showing Ramos cells stained with ab108978 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108978, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-Tcl1 antibody [EPR3949] (ab108978) at 1/1000 dilutionLane 1 : Daudi cell lysateLane 2 : Ramos cell lysatesLysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-goat. at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab108978.

ab108978 showing negative staining in Skeletal muscle tissue.

ab108978 showing positive staining in Normal spleen tissue.

ab108978 showing negative staining in Normal brain tissue.

ab108978 showing negative staining in Normal kidney tissue.

ab108978 showing negative staining in Normal stomach tissue.