Anti-TDP43 antibody
| Name | Anti-TDP43 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab41881 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ICC/IF ICC/IF IHC-F |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human TARDBP |
| Description | Rabbit Polyclonal |
| Gene | TARDBP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-TDP43 antibody (ab41881) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 8 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate - tumor cell line (ab29309)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab41881 stained human Hek293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab41881, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Lane 1: Marker, Lane 2: 293T Total Lysate (Input), Lane 3: 293T supernatant (Flow through), LAne 4: Pull down (IP).See Abreview
ab41881 staining TARDBP in murine spinal cord by Immunohistochemistry (PFA perfusion fixed frozen sections).Tissues fixed by 4% paraformaldehyde in phosphate buffer (pH 7.4) were cryoprotected by 30% sucrose in PBS (pH 7.4). Frozen sections (10 µm) of spinal cord tissues from three or more transgenic mice, respectively, were processed for immunofluorescence using ab41881. An Alexa Fluor 594 was used as secondary antibodies to visualize protein. Scale bar 20 µm.TARDBP is localized in the nucleus and forms intranuclear granular structures, arrowheads.
Product References
Metabolism and mis-metabolism of the neuropathological signature protein TDP-43. - Metabolism and mis-metabolism of the neuropathological signature protein TDP-43.
Huang CC, Bose JK, Majumder P, Lee KH, Huang JT, Huang JK, Shen CK. J Cell Sci. 2014 Jul 15;127(Pt 14):3024-38.
Neurotoxic 43-kDa TAR DNA-binding protein (TDP-43) triggers - Neurotoxic 43-kDa TAR DNA-binding protein (TDP-43) triggers
Braun RJ, Sommer C, Carmona-Gutierrez D, Khoury CM, Ring J, Buttner S, Madeo F. J Biol Chem. 2011 Jun 3;286(22):19958-72.
Altered distributions of Gemini of coiled bodies and mitochondria in motor - Altered distributions of Gemini of coiled bodies and mitochondria in motor
Shan X, Chiang PM, Price DL, Wong PC. Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16325-30. doi:
Variations of protein levels in human amniotic fluid stem cells CD117/2 over - Variations of protein levels in human amniotic fluid stem cells CD117/2 over
Chen WQ, Siegel N, Li L, Pollak A, Hengstschlager M, Lubec G. J Proteome Res. 2009 Nov;8(11):5285-95.