TARDBP

Gene Symbol TARDBP
Entrez Gene 23435
Alt Symbol ALS10, TDP-43
Species Human
Gene Type protein-coding
Description TAR DNA binding protein
Other Description TAR DNA-binding protein 43|TAR DNA-binding protein-43
Swissprots E2PU12 A4GUK4 Q13148 B4DJ45 Q6FI92 Q53H27 A4GUK5 Q96DJ0 A4GUK6 B2R629
Accessions CBF67564 CBI68017 CBU92437 EAW71669 EAW71670 EAW71671 EAW71672 EAW71673 Q13148 AF311304 AAG47782 AK222754 BAD96474 AK295920 BAG58707 AK299454 BAG61425 AK312416 BAG35326 AL045296 AL050265 CAB43367 AM392916 CAL37794 BC001487 BC059955 BC071657 AAH71657 BC095435 AAH95435 BI464234 BM716882 CD703666 CR533534 CAG38565 EF434181 ABO32290 EF434182 ABO32291 EF434183 ABO32292 EU446942 ABZ92471 HQ628633 AER93383 HQ628634 AER93384 HQ628635 AER93385 HQ628636 AER93386 HQ628637 AER93387 U23731 AAA70033 XR_946596 XR_946597 NM_007375 NP_031401
Function DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR. {ECO:0000269|PubMed:11285240, ECO:0000269|PubMed:17481916}.
Subcellular Location Nucleus {ECO:0000269|PubMed:17023659, ECO:0000269|PubMed:17481916}. Note=In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.
Tissue Specificity Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.

TDP43 (A260) Antibody - 3449 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells and rat brain using TDP43 (A260) Antibody.

TDP43 (G400) Antibody - 3448 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells and rat brain using TDP43 (G400) Antibody.

TARDBP (H-8) - sc-376532 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TARDBP (H-8): sc-376532. Western blot analysis of TARDBP expression in Jurkat whole cell lysate.

TARDBP (E-10) - sc-376311 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TARDBP (E-10): sc-376311. Western blot analysis of TARDBP expression in Hep G2 (A) and Jurkat (B) whole cell lysates.

TARDBP (H-89) - sc-292299 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

TARDBP (S-14) - sc-131799 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TARDBP (P-24) - sc-102127 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
TARDBP (P-24): sc-102127. Western blot analysis of TARDBP expression in Jurkat whole cell lysate.

TARDBP (41-7.1) - sc-100871 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
TARDBP (41-7.1): sc-100871. Western blot analysis of TARDBP expression in A-431 whole cell lysate.

Anti-TDP43 (phospho S409 + S410) antibody [1D3] - ab184683 from Abcam

Prices $370.00
Sizes 50 µl
Host Rat
Clonality Monoclonal
Clone 1D3

Anti-TDP43 antibody - ab41881 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TDP43 antibody (ab41881) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 8 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate - tumor cell line (ab29309)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-TDP43 antibody - ab154047 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TDP43 antibody (ab154047) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : H1299 whole cell lysateLane 3 : HeLa whole cell lysateLysates/proteins at 30 µg per lane.

Anti-TDP43 antibody [2E2-D3] - ab57105 from Abcam

Prices $390.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2E2-D3
TARDBP antibody (ab57105) used in immunofluorescence at 10ug/ml on HeLa cells.

Anti-TDP43 antibody [3H8] - ab104223 from Abcam

Prices $379.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 3H8
Overlay histogram showing JEG3 cells stained with ab104223 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab104223, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in JEG3 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-TDP43 antibody [EPR5810] - ab109535 from Abcam

Prices $99.00, $379.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5810
All lanes : Anti-TDP43 antibody [EPR5810] (ab109535) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : K562 cell lysateLane 4 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution

Anti-TDP43 antibody [EPR5810] (Alexa Fluor® 488) - ab193842 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5810
ab193842 staining TDP43 in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab193842 at a 1/250 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-TDP43 antibody [EPR5810] (HRP) - ab193850 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5810
IHC image of TARDBP staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab193850 at 1/500 dilution, for 15 mins at room temperature.DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-TDP43 antibody [EPR5811] - ab133547 from Abcam

Prices $99.00, $379.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5811
All lanes : Anti-TDP43 antibody [EPR5811] (ab133547) at 1/10000 dilutionLane 1 : HepG2 cell line lysateLane 2 : Hela cell line lysateLane 3 : K562 cell line lysateLane 4 : 293T cell line lysateLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit at 1/2000 dilution

Anti-TDP43 antibody [GT733] - N-terminal - ab184645 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone GT733
All lanes : Anti-TDP43 antibody [GT733] - N-terminal (ab184645) at 1/1000 dilutionLane 1 : 293T whole cell lysate/extractLane 2 : A431 whole cell lysate/extractLane 3 : HeLa whole cell lysate/extractLane 4 : HepG2 whole cell lysate/extractLysates/proteins at 30 µg per lane.

Anti-TDP43 antibody [K1B8] - ab115952 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone K1B8
ab115952 at 10 ug/ml staining TARDBP in formalin fixed Human lung tissue by immunohistochemistry. Also stained with biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-TDP-43 / TARDBP Antibody - LS-C56457 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TDP-43 / TARDBP Antibody (aa1-30) IHC-plus™ - LS-B10176 from LifeSpan Bioscience

Prices $395.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-TDP-43 / TARDBP antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-TDP-43 / TARDBP Antibody (clone 2E2-D3) IHC-plus™ - LS-B4521 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2E2-D3
Anti-TARDBP antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4521 concentration 5 ug/ml.

Anti-TDP-43 / TARDBP Antibody (C-Terminus) IHC-plus™ - LS-B4917 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-TARDBP antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4917 concentration 10 ug/ml.

Anti-TDP-43 / TARDBP Antibody (Internal) IHC-plus™ - LS-B2223 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-TARDBP antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2223 concentration 5 ug/ml.

Anti-TDP-43 / TARDBP Antibody - LS-C331331 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded rat brain lung using TARDBP antibody at dilution of 1:200 (400x lens).
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