Anti-TDP43 antibody [2E2-D3]
| Name | Anti-TDP43 antibody [2E2-D3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab57105 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2E2-D3 |
| Applications | FC WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein corresponding to Human TDP43 aa 1-261 |
| Description | Mouse Monoclonal |
| Gene | TARDBP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
TARDBP antibody (ab57105) used in immunofluorescence at 10ug/ml on HeLa cells.
TARDBP antibody (ab57105) at 1ug/lane + A-431 cell lysate at 25ug/lane.
![Overlay histogram showing HeLa cells stained with ab57105 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57105, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/9578_TARDBP-Primary-antibodies-ab57105-1.jpg)
Overlay histogram showing HeLa cells stained with ab57105 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57105, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Mitochondrial dysfunction and decrease in body weight of a transgenic knock-in - Mitochondrial dysfunction and decrease in body weight of a transgenic knock-in
Stribl C, Samara A, Trumbach D, Peis R, Neumann M, Fuchs H, Gailus-Durner V, Hrabe de Angelis M, Rathkolb B, Wolf E, Beckers J, Horsch M, Neff F, Kremmer E, Koob S, Reichert AS, Hans W, Rozman J, Klingenspor M, Aichler M, Walch AK, Becker L, Klopstock T, Glasl L, Holter SM, Wurst W, Floss T. J Biol Chem. 2014 Apr 11;289(15):10769-84.
HIV-1 replication in human immune cells is independent of TAR DNA binding protein - HIV-1 replication in human immune cells is independent of TAR DNA binding protein
Nehls J, Koppensteiner H, Brack-Werner R, Floss T, Schindler M. PLoS One. 2014 Aug 15;9(8):e105478.
Efficient conditional and promoter-specific in vivo expression of cDNAs of choice - Efficient conditional and promoter-specific in vivo expression of cDNAs of choice
Schebelle L, Wolf C, Stribl C, Javaheri T, Schnutgen F, Ettinger A, Ivics Z, Hansen J, Ruiz P, von Melchner H, Wurst W, Floss T. Nucleic Acids Res. 2010 May;38(9):e106.
CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent - CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent
Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. Nucleic Acids Res. 2010 Nov;38(20):7273-85.
Phosphorylation promotes neurotoxicity in a Caenorhabditis elegans model of - Phosphorylation promotes neurotoxicity in a Caenorhabditis elegans model of
Liachko NF, Guthrie CR, Kraemer BC. J Neurosci. 2010 Dec 1;30(48):16208-19.