All lanes : Anti-TDP43 antibody [GT733] - N-terminal (ab184645) at 1/1000 dilutionLane 1 : 293T whole cell lysate/extractLane 2 : A431 whole cell lysate/extractLane 3 : HeLa whole cell lysate/extractLane 4 : HepG2 whole cell lysate/extractLysates/proteins at 30 µg per lane.
Immunofluorescent analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labeling TARDBP using ab184645 diluted 1/500 (green). Hoechst 33342 staining (blue).
All lanes : Anti-TDP43 antibody [GT733] - N-terminal (ab184645) at 1/1000 dilutionLane 1 : Whole cell lysate/extract of 293T cellsLane 2 : Whole cell lysate/extract of TARDBP shRNA-transfected 293T cellsLysates/proteins at 30 µg per lane.