All lanes : Anti-TEAD4 antibody (ab110796) at 1 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 2 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell LysateLane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell LysateLane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLane 6 : HCT 116 (Human Colorectal Carcinoma) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.